ABSTRACT
Assessments of cell reactions such as motility, orientation and activation to the topography of the substratum will assist with the fabrication of a proper implantable scaffold for future tissue engineering applications. The current challenge is to analyze the orientation effect of elecrospun nanofibers of poly [epsilon-caprolactone] [PCL] on viability and proliferation of mouse embryonic stem cells [mESCs]. In this experimental study, we used the electrospinning method to fabricate nanofibrous PCL scaffolds. Chemical and mechanical characterizations were specified by the contact angle and tensile test. O2 plasma treatment was used to improve surface hydrophilicity. We used the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide [MTT] assay to evaluate mESCs adhesion and proliferation before and after surface modification. The influence of the orientation of the nanofibers on mESCs growth was evaluated by scanning electron microscopy [SEM]. Statistical analysis was performed using one-way analysis of variance [ANOVA] With differences considered statistically significant at p